General Notes
This product (Catalog No. DPABH-28670) is a rabbit-derived polyclonal antibody raised against human CS, targeting the amino acid region 103–394 of the protein. This antibody exhibits cross-reactivity with Human CS from mouse and rat. It has been affinity purified by Protein A. Citrate synthase (CS; EC 2.3.3.1) is a pivotal enzyme in cellular metabolism, catalyzing the condensation of acetyl-coenzyme A (acetyl-CoA) and oxaloacetate to form citrate and coenzyme A, thereby initiating the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle or citric acid cycle. This reaction represents the first and rate-limiting step of the TCA cycle, linking carbohydrate, lipid, and protein catabolism to generate reducing equivalents (NADH and FADH₂) for ATP production via oxidative phosphorylation. Encoded by the nuclear gene CS on human chromosome 12q13.3, the enzyme is synthesized in the cytosol as a precursor with an N-terminal mitochondrial targeting sequence and imported into the mitochondrial matrix, where it functions as a homodimer (or higher-order oligomer in some species) with a molecular mass of approximately 100 kDa.
DPABH-28670 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-28670 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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