General Notes
This product (Catalog No. DPABH-06361) is a rabbit-derived polyclonal antibody raised against human CISD2, targeting the amino acid region 61–135 of the protein. This antibody exhibits cross-reactivity with Human CISD2 from mouse and rat. It has been affinity purified by Protein A. CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on the endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates the autophagy program by interacting with BCL2, contributing to antagonizing BECN1-mediated cellular autophagy at the endoplasmic reticulum.
DPABH-06361 is suitable for use in Western blot (WB) (1:2,000–1:10,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:50–1:500), IF/ICC (1:200–1:800), flow cytometry (FC) (0.25 μg per 10^6 cells in a 100 μl suspension) applications.
DPABH-06361 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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