Anti-CHMP3 (N-terminal) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Mouse; Rat; Human

Immunogen

Synthetic peptide from the N terminal of human VPS24 conjugated to an immunogenic carrier protein.

Conjugate

Application Notes

IHC-P: Use at a concentration of 10 - 50 μg/ml (antigen retrieval is recommended). IHC-Fr: Use at a concentration of 10 - 50 μg/ml. WB: Use at a concentration of 10 - 50 μg/ml. Predicted molecular weight: 25 kDa.

Alternative Names

CHMP3; charged multivesicular body protein 3; NEDF; VPS24; CGI-149; 25.1 protein; CHMP family, member 3; chromatin-modifying protein 3; vacuolar protein sorting 24 homolog; neuroendocrine differentiation factor; vacuolar protein sorting-associated protein

Entrez Gene ID

UniProt ID

Antigen Description

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.

Pathway

Budding and maturation of HIV virion; ESCRT-III complex; Endocytosis; Endosomal Sorting Complex Required For Transport (ESCRT)