Anti-CD1B monoclonal antibody, clone C-C6 [FITC]

Background: The differential diagnosis of inflammatory skin diseases is largely based on the patient's history anti the morphological analysis of the skin lesion. Laboratory data, such as serum IgE-level and prick and patch tests, may be helpful but do not assess individual lesions. The assumption of our approach is that each individual lesion is associated with a specific microenvironment and that the immunophenotype of the two epidermal dendritic cell populations, Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDEC), reflects this environment in a disease-specific manner. Methods: A now cytometric micromethod was developed to directly analyze individual inflammatory human skin lesions. Crude epidermal single cell suspensions were prepared by trypsinization, stained for three-color analysis With different monoclonal antibodies and the vital stain 7-amino-actinomycin-D, and finally analyzed on a single laser equipped FACScan flow cytometer. Results: With a limited set of cell surface markers, such as Fc epsilon RI, Fc gamma RII/CD32, CD1b and CD36, highly specific diagnostic criteria for atopic dermatitis and inflamed human skin could be established. Conclusions: Phenotyping of epidermal dendritic cells is a useful procedure helpful in differential diagnosis of inflammatory skin diseases. Cytometry 37:147-155, 1999. (C) 1999 Wiley-Liss, Inc.

Human gamma delta T cells expressing the V delta 3 TCR make up a minor lymphocyte subset in blood but are enriched in liver and in patients with some chronic viral infections and leukemias. We analyzed the frequencies, phenotypes, restriction elements, and functions of fresh and expanded peripheral blood V delta 3 T cells. V delta 3 T cells accounted for similar to 0.2% of circulating T cells, included CD4(+), CD8(+), and CD4(-)CD8(-) subsets, and variably expressed CD56, CD161, HLA-DR, and NKG2D but neither NKG2A nor NKG2C. V delta 3 T cells were sorted and expanded by mitogen stimulation in the presence of IL-2. Expanded V delta 3 T cells recognized CD1d but not CD1a, CD1b, or CD1c. Upon activation, they killed CD1d(+) target cells, released Th1, Th2, and Th17 cytokines, and induced maturation of dendritic cells into APCs. Thus, V delta 3 T cells are glycolipid-reactive T cells with distinct Ag specificities but functional similarities to NKT cells. The Journal of Immunology, 2013, 191: 30-34.