Anti-CCNB1 monoclonal antibody (DCABH-9535) Knockout Validated

Mouse anti-Human CCNB1 monoclonal antibody for SDS-PAGE, ICC/IF, IHC(PFA fixed), FC, WB, IHC-FoFr, IHC-P, IHC-Fr

Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
W263
Species Reactivity
Human
Immunogen
His-tagged Hamster Cyclin B1 expressed in bacteria, harvested from inclusion bodies, extracted with 6M guanidine HCl and purified on Nickel beads.
Conjugate
Unconjugated

Applications


Application Notes
SDS-PAGE: 1/1500; IHC: 1/400; WB: 1 μg/ml; IHC-P: 5 μg/ml; IHC-Fr: 1 - 2 μg/ml.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
CCNB1; cyclin B1; CCNB; G2/mitotic-specific cyclin-B1; G2/mitotic specific cyclin B1; G2/mitotic-specific cyclin B1
Entrez Gene ID
UniProt ID

Product Background


Pathway
APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem;

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Quintana, AM; Liu, F; et al. Identification and Regulation of c-Myb Target Genes in MCF-7 Cells. BMC CANCER 11:-(2011).
Cattoretti, G; Buttner, M; et al. Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells. BLOOD 107:3967-3975(2006).

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