General Notes
This product (Catalog No. DPABH-27259) is a rabbit-derived polyclonal antibody raised against human CASP1, targeting the amino acid region 120–297 of the protein. It has been affinity purified by Protein A. Caspase-1, also known as interleukin-1β converting enzyme (ICE), is an evolutionarily conserved cysteine protease that functions as a key mediator in the innate immune response by processing pro-inflammatory cytokines and inducing pyroptosis. Encoded by the human CASP1 gene located on chromosome 11q22.3, it is synthesized as an inactive 45 kDa zymogen consisting of a CARD-containing prodomain, a large p20 subunit, and a small p10 subunit. Upon activation within inflammasome complexes, caspase-1 undergoes autoproteolytic cleavage to form an active heterotetrameric enzyme (p20)₂(p10)₂, characterized by a catalytic dyad of Cys285 and His237 that enables substrate specificity for aspartate residues.
DPABH-27259 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:500–1:2,000) applications.
DPABH-27259 is supplied as a liquid formulation in Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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