Anti-CAMK2B (aa 405-502) polyclonal antibody

In our previous study using iTRAQ technique we found that the level of calmodulin-dependent protein kinase 2b (Camk2b) was lower in rats with hyperhomocysteinemia. We presumed that Camk2b might be involved in homocysteine-induced apoptosis and tried to explore its role in this study through the transfection with Camk2b gene. Results showed that neurons of HHcy group had lower activity measured by MTT, higher percentage of apoptotic neurons, lower expression levels of Camk2b mRNA and protein than those in normal group. Neurons with overexpression of Camk2b (Camk2b group) had lower percentage of apoptosis and higher activity than those in control group. After exposure to 2-Methoxyestradiol, the activity of neurons with overexpression of Camk2b was suppressed with more apoptotic cells observed. The expressions of BCL2, eNOS, EP300 and EPO were all elevated at both mRNA and protein levels in neurons of CamK2b group compared with other three groups. Thus, Camk2b protects neurons from Homocysteine-induced apoptosis with the involvement of HIF-1 alpha signal pathway.

Objectives: Obesity is associated with breast cancer. Our aim was to study gene expression differences of Wnt pathway in breast cancer patients when they have different BMI. Methods: According to established WHO criteria, breast cancer patients were divided into normal, overweight, obese three groups. We extracted Wnt pathway gene expression data of the three groups, perform one-way ANOVA and LSD-t test. According to the significance level, we confirm whether there exist differences in gene expression. Rresults: In breast cancer patients with different degrees of obesity, some genes of Wnt pathway showing differential expression, a total of fifteen, are as follows: CTBP2, CREBBP, ROCK2, BAMBI, PSEN1, FZD7, MAP3K7, CUL1, PPP3CB, NFATC3, BTRC, NLK, FRAT1, CSNK1E, and CAMK2B. Conclusions: Differential gene expression of Wnt pathway in the three groups is closely related to the degree of obesity in breast cancer patients. These genes are widely distributed in the Wnt pathway.