Specifications
Species Reactivity
Mouse; Rat; Rabbit; Chicken; Guinea pig; Human; Pig
Immunogen
Full length native protein (purified) corresponding to Rabbit Calcium channel L type DHPR alpha 2 subunit.
Applications
Application Notes
Flow Cyt: 1/200; ICC: 1/250; IHC-P: 1/500; IHC-Fr: 1/500; WB: 1/500.
Target
Alternative Names
CACNA2D1; calcium channel, voltage-dependent, alpha 2/delta subunit 1; voltage-dependent calcium channel subunit alpha-2/delta-1; voltage-gated calcium channel subunit alpha-2/delta-1; dihydropryridine-sensitive calcium channel alpha-2 subunit;
Product Background
Antigen Description
The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.
Citations
Publication ()
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Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior
Jin J, Bhatti DL, Lee KW, Medrihan L, Cheng J, Wei J, Zhong P, Yan Z, Kooiker C, Song C, Ahn JH, Obermair GJ, Lee A, Gresack J, Greengard P, Kim Y
Applications: WB
Reactive species: Mouse
"Abstract: Genetic polymorphisms of the L-type voltage-gated calcium channel (VGCC) are associated with psychiatric disorders including major depressive disorder. Alterations of S100A10 (p11) level are also implicated in the etiology of major depressive disorder. However, the existence of an endogenous regulator in the brain regulating p11, L-type VGCC, and depressive behavior has not been known. Here we report that Ahnak, whose function in the brain has been obscure, stabilizes p11 and Anxa2 proteins in the hippocampus and prefrontal cortex in the rodent brain. Protein levels of Ahnak, p11, and Anxa2 are highly and positively correlated in the brain. Together these data suggest the existence of an Ahnak/p11/Anxa2 protein complex. Ahnak is expressed in p11-positive as well as p11-negative neurons. Ahnak, through its N-terminal region, scaffolds the L-type pore-forming α1 subunit and, through its C-terminal region, scaffolds the β subunit of VGCC and the p11/Anxa2 complex. Cell surface expression of the α1 subunits and L-type calcium current are significantly reduced in primary cultures of Ahnak knockout (KO) neurons compared to wild-type controls. A decrease in the L-type calcium influx is observed in both glutamatergic neurons and parvalbumin (PV) GABAergic interneurons of Ahnak KO mice. Constitutive Ahnak KO mice or forebrain glutamatergic neuron-selective Ahnak KO mice display a depression-like behavioral phenotype similar to that of constitutive p11 KO mice. In contrast, PV interneuron-selective Ahnak KO mice display an antidepressant-like behavioral phenotype. Our results demonstrate L-type VGCC as an effector of the Ahnak/p11/Anxa2 complex, revealing a novel molecular connection involved in the control of depressive behavior."
Article snippet: Immunoblotting was performed with a standard protocol using the following antibodies: anti-Ahnak (*, *, 1:50,000 or *, *, 1:10,000), anti-p11 (goat polyclonal, *, *, 1:1,000), anti-Anxa2 (mouse monoclonal, *, *, 1:1000), anti-Gapdh (mouse monoclonal, *, *, 1:5,000), anti-Cav1.2 (rabbit polyclonal, *, *, 1:2,500), anti-Cav1.3 (rabbit polyclonal, *, 1:1000), anti-Flag (mouse monoclonal, *, *, 1:1,000), anti-V5 (mouse monoclonal, *, *, 1:1000), anti-HA (rat monoclonal, *, *, 1:1,000), anti-β1 subunit (mouse monoclonal, *, *, 1:1000), anti-β2a subunit (rabbit polyclonal antibody, *, 1:1,000), anti-β3 subunit (rabbit polyclonal antibody, *, 1:1,000), anti-β4 subunit (mouse monoclonal, *, *, 1:1000), anti-α2δ1 subunit (mouse monoclonal, DCABH-8461, Creative Diagnostics, 1:1000). 
Figure 1. Protein levels of two α1 subunits, four β subunits, α2δ1 subunit and Gapdh in the PFC. 
Figure 2. Protein levels of two α1 subunits, four β subunits, α2δ1 subunit and Gapdh in the hippocampus.
COA
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