Anti-BRAF monoclonal antibody (CAB-7280RH)

Rabbit Anti-BRAF monoclonal antibody for WB, IHC, IP, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TV45-15
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000; FC: 1:50-1:100; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Fig4: Flow cytometric analysis of Hela cells with B Raf antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B Raf proto oncogene, serine/threonine kinase; B RAF1
Entrez Gene ID

Citations


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References


Pang, NKB; Nga, ME; et al. KRAS and BRAF mutation analysis can be reliably performed on aspirated cytological specimens of metastatic colorectal carcinoma. CYTOPATHOLOGY 22:358-364(2011).
Er, TK; Chen, CC; et al. Clinical relevance of KRAS mutations in codon 13: Where are we?. CANCER LETTERS 343:1-5(2014).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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