Rabbit Anti-Human BACH1 (a.a. 501-600) Polyclonal antibody

Name: Rabbit Anti-Human BACH1 (a.a. 501-600) Polyclonal antibody
SKU: DPABH-26524
Rating: 5 (1 reviews)

Rabbit Anti-Human BACH1 (a.a. 501-600) Polyclonal antibody for WB, IHC-P, IHC-F, IF, FC

Write a review

COA

SDS

Datasheet

Key features and details

Target: BACH1
Host: Rabbit
Application: WB, IHC-P, IHC-F, IF, FC
Species Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat; Chicken; Dog; Pig; Cow; Horse; Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human BACH1: 501-600/736

Conjugate

Unconjugated

Applications

Application Notes

WB: 1:500-2000
IHC-P: 1:100-500
IHC-F: 1:100-500
IF: 1:100-500
Flow-Cyt: 2 μg/Test

General Notes

This product (Catalog No. DPABH-26524) is a rabbit-derived IgG polyclonal antibody raised against human BACH1, targeting the amino acid region 501–600 of the protein. This antibody exhibits cross-reactivity with BACH1 from mouse, rat, chicken, dog, pig, cow, horse, and rabbit. It has been affinity purified by Protein A. This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.
DPABH-26524 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC-P (1:100–1:500), IHC-F (1:100–1:500), IF (immunofluorescence) (1:100–1:500), flow cytometry (FC) (2 μg/test) applications.
DPABH-26524 is supplied as a liquid formulation in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Alternative Names

BTB Domain And CNC Homolog 1; BTB And CNC Homology 1, Basic Leucine Zipper Transcription Factor 1; Basic Region Leucine Zipper Transcriptional Regulator BACH1; Transcription Regulator Protein BACH1; BTB And CNC Homolog 1; BACH-1; BTBD24; HA2303; BACH1_HUMAN

Entrez Gene ID

571

UniProt ID

O14867

Citations

Have you cited DPABH-26524 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Mechanisms of Cell Protection by Heme Oxygenase-1

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY

Authors: Gozzelino, Raffaella; Jeney, Viktoria; Soares, Miguel P.

Abstract

Heme oxygenases (140) catabolize free heme, that is, iron (Fe) protoporphyrin (IX), into equimolar amounts of Fe2+, carbon monoxide (CO), and biliverdin. The stress-responsive HO-1 isoenzyme affords protection against programmed cell death. The mechanism underlying this cytoprotective effect relies on the ability of HO-1 to catabolize free heme and prevent it from sensitizing cells to undergo programmed cell death. This cytoprotective effect inhibits the pathogenesis of a variety of immune-mediated inflammatory diseases.

Functional role of BTB and CNC Homology 1 gene in pancreatic cancer and its association with survival in patients treated with gemcitabine

THERANOSTICS

Authors: Huang, Xudong; Zheng, Jian; Li, Jun; Che, Xu; Tan, Wenle; Tan, Wen; Shao, Mingming; Cheng, Xinxin; Du, Zhongli; Zhao, Yanjie; Wang, Chengfeng; Wu, Chen; Lin, Dongxin

Abstract

Genetic variation (rs372883C/T) in the 3'-untranslated region of BTB and CNC homology 1 (BACH1) has been associated with pancreatic ductal adenocarcinoma (PDAC) risk in our previous genome-wide association study; however, the action roles of this genetic variation in PDAC remains unknown. Methods: BACH1 expression was measured by quantitative real-time PCR, Western blot and immunohistochemistry. The effects of BACH1 on cell proliferation and sensitivity to gemcitabine were examined by alteration of BACH1 expression in PDAC cells. Angiogenesis was determined in vitro using a human umbilical vein endothelial cell model. Reporter gene assays were conducted to compare the effects of microRNA-1257 on rs372883 variation. The associations between rs372883 variants and survival time in patients treated with gemcitabine were estimated by logistic regression. Results: We found substantially lower BACH1 expression in PDAC compared with normal pancreatic tissues and the rs372883T allele had significantly lower BACH1 levels than the rs372883C allele in both tumor and normal tissues. Knockdown of BACH1 expression provoked proliferation of PDAC cells and angiogenesis, which might result from upregulation of hemeoxygenase-1 that evokes oncogenic AKT and ERK signaling. The rs372883T>C change inhibits interaction of BACH1 with microRNA-1257, resulting in increased BACH1 expression. PDAC patients with the rs372883T allele were more resistant to gemcitabine and had shorter survival time compared with those with the rs372883C allele. Conclusion: These results shed light on the mechanism underlying the associations of BACH1 rs372883 variation with risk of developing PDAC and differential gemcitabine sensitivity in patients.