General Notes
This product (Catalog No. DPABH-15206) is a rabbit-derived polyclonal antibody raised against human ALAS1, targeting the amino acid region 217–640 of the protein. It has been affinity purified by Protein A. ALAS1 is a human gene that encodes 5-aminolevulinate synthase 1, a mitochondrial enzyme which catalyzes the rate-limiting first step of heme biosynthesis by condensing glycine and succinyl-CoA to form 5-aminolevulinic acid (ALA), the universal precursor for heme and porphyrin synthesis. This housekeeping isoform of the enzyme, distinct from the erythroid-specific ALAS2, is essential for maintaining heme production in non-erythroid tissues, supporting functions such as oxygen transport, electron transfer, and detoxification across various cell types. Expressed predominantly in the liver and adrenal glands, ALAS1 is nuclear-encoded but functions within the mitochondrial matrix, where its activity is tightly regulated by heme levels—high heme concentrations promote degradation of the mature enzyme, while low levels enhance its stability and expression. Dysregulation of ALAS1, rather than mutations, contributes to acute hepatic porphyrias through excessive heme precursor accumulation; it is targeted by the FDA-approved siRNA therapy givosiran for acute intermittent porphyria. Structurally, human ALAS1 features a pyridoxal 5'-phosphate-dependent catalytic mechanism; crystal structures of related ALAS isoforms, such as ALAS2, have revealed adaptations unique to eukaryotic heme pathways.
DPABH-15206 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-15206 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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