Recombinant human ATP6V1F protein; Source: E. coli derived, PGEX-4T; Tag: GST; Domain: 1-119 aa of BC107854
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:2000 IHC: 1:20-1:200
General Notes
This product (Catalog No. DPABH-05412) is a rabbit-derived polyclonal antibody raised against human ATP6V1F, targeting the amino acid region 1–119 of the protein. This antibody exhibits cross-reactivity with Human ATP6V1F from mouse. It has been affinity purified by Protein A. ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa. DPABH-05412 is suitable for use in Western blot (WB) (1:500–1:2,000), IHC (1:20–1:200) applications. DPABH-05412 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
Target
Alternative Names
ATP6S14; ATP6V1F; V ATPase 14 kDa subunit; V ATPase subunit F; V type proton ATPase subunit F; Vacuolar proton pump subunit F; VATF; Vma7
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