General Notes
This product (Catalog No. DPABH-08519) is a rabbit-derived polyclonal antibody raised against human ARHGAP25, targeting the amino acid region 1–347 of the protein. This antibody exhibits cross-reactivity with Human ARHGAP25 from mouse and rat. It has been affinity purified by Protein A. ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.
DPABH-08519 is suitable for use in Western blot (WB) (1:500–1:2,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:20–1:200), IF/ICC (1:50–1:500) applications.
DPABH-08519 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.