Anti-ARF1 monoclonal antibody, clone 3G8E3

Clone

3G8E3

Species Reactivity

Human

Immunogen

Recombinant full length protein corresponding to Human ARF1 aa 1-181.Sequence: MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIG FNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERV NEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRH RNWYIQATCATSGDGLYEGLDWL

Conjugate

Application Notes

IHC-P: 1/50 - 1/100; WB: 1/100 - 1/2000.

Alternative Names

ARF1; ADP-ribosylation factor 1;

Entrez Gene ID

UniProt ID

Antigen Description

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.

Pathway

Arf1 pathway, organism-specific biosystem; Arf6 downstream pathway, organism-specific biosystem; COPI Mediated Transport, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; Clathrin derived vesicle budding, organism-specific biosystem; Disease, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem;