Recombinant human AP3B2 protein; Source: E. coli derived, PET28a; Tag: 6*His; Domain: 650-1082 aa of BC093739
Conjugate
Unconjugated
Applications
Application Notes
WB: 1:500-1:1000 IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate
General Notes
This product (Catalog No. DPABH-24740) is a rabbit-derived polyclonal antibody raised against human AP3B2, targeting the amino acid region 650–1082 of the protein. This antibody exhibits cross-reactivity with Human AP3B2 from mouse and rat. It has been affinity purified by Protein A. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3B2 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. DPABH-24740 is suitable for use in Western blot (WB) (1:500–1:1,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate) applications. DPABH-24740 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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