Anti-ANAPC1 PAb (DPABH-25647)

Rabbit anti-Human Apc1 (phospho S355) polyclonal antibody for WB, ELISA Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human
Immunogen
Synthetic peptide: SRAHSpPALG conjugated to KLH, corresponding to amino acids 351-359 of Human Apc1.
Conjugate
Unconjugated

Applications


Application Notes
ELISA: 1/10000 - 1/40000. WB: 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). Tested in peptide ELISA. Product has been tested against 0.1 μg of the phosphorylated and non-phosphorylated forms of the immunizing peptide. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
ANAPC1; anaphase promoting complex subunit 1; APC1; MCPR; TSG24; anaphase-promoting complex subunit 1
Entrez Gene ID
UniProt ID

Product Background


Pathway
APC/C complex; APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1

Citations


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References


Kraft, C; Herzog, F; et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation. EMBO JOURNAL 22:6598-6609(2003).
Chen, PF; Harcum, SW; et al. Differential display identifies genes in Chinese hamster ovary cells sensitive to elevated ammonium. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 141:349-359(2007).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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