Anti-ANAPC1 PAb (DPABH-25647)
Rabbit anti-Human Apc1 (phospho S355) polyclonal antibody for WB, ELISA
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Synthetic peptide: SRAHSpPALG conjugated to KLH, corresponding to amino acids 351-359 of Human Apc1.
ELISA: 1/10000 - 1/40000. WB: 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). Tested in peptide ELISA. Product has been tested against 0.1 μg of the phosphorylated and non-phosphorylated forms of the immunizing peptide. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
ANAPC1; anaphase promoting complex subunit 1; APC1; MCPR; TSG24; anaphase-promoting complex subunit 1
APC/C complex; APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
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Zhu, F; Zhang, M; et al. DNA polymerase zeta: new insight into eukaryotic mutagenesis and mammalian embryonic development. WORLD JOURNAL OF GASTROENTEROLOGY 9:1165-1169(2003).
Golan, A; Yudkovsky, Y; et al. The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk. JOURNAL OF BIOLOGICAL CHEMISTRY 277:15552-15557(2002).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.