General Notes
This product (Catalog No. DPABH-11575) is a rabbit-derived polyclonal antibody raised against human ADH5, targeting the amino acid region 1–374 of the protein. It has been affinity purified by Protein A. ADH5 is a protein-coding gene in humans, officially named alcohol dehydrogenase 5 (class III), chi polypeptide, that encodes a zinc-containing dimeric enzyme essential for metabolizing various substrates, including long-chain primary alcohols and formaldehyde adducts. Located on chromosome 4q23, the gene spans approximately 17.8 kb with nine exons and is ubiquitously expressed across tissues, with highest levels in the endometrium and kidney. The encoded protein, also known as glutathione-dependent formaldehyde dehydrogenase (GSNOR) or S-nitrosoglutathione reductase, primarily catalyzes the NAD+-dependent oxidation of S-(hydroxymethyl)glutathione—a toxic adduct formed from formaldehyde and glutathione—into S-formylglutathione, thereby detoxifying formaldehyde, a potent irritant and genotoxin. Unlike other alcohol dehydrogenases (e.g., classes I and II), ADH5 exhibits negligible activity toward ethanol but high specificity for long-chain alcohols and omega-hydroxy fatty acids, contributing to cellular protection against oxidative and nitrosative stress through regulation of S-nitrosothiols and nitric oxide signaling.
DPABH-11575 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-11575 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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