Human brain homogenate of multiple sclerosis plaques obtained at autopsy
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
NS-1
Clone
H-4-6
Cell Line Description
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes a novel intermediate filament associated protein (MW 100000) with similarity to alpha actinin. This antibody intensely stains astroglia and related macroglia in cryostat sections of human and rat central nervous system tissue.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 0.5x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Murata, J; Tokunaga, A; et al. Mapping of notch activation during cochlear development in mice: Implications for determination of prosensory domain and cell fate diversification. JOURNAL OF COMPARATIVE NEUROLOGY 497:502-518(2006).
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COA
Certificate of Analysis
