Ampicillin ELISA Kit

Sample

Animal tissue (muscle, liver, fish and shrimp), honey and milk

Species Reactivity

N/A

Detection Method

cELISA

Intended Use

This kit can be used in quantitative and qualitative analysis of ampicillin residue in animal tissue (muscle, liver, fish and shrimp), honey and milk, etc.

Contents of Kit

1. Microtiter plate with 96 wells coated with antigen
2. Standard solutions. (1ml×6 bottles): 0 ppb, 0.2ppb, 0.6ppb, 1..8ppb, 5.4ppb, 16.2ppb
3. Spiking standard solution: 1ml, 1ppm
4. Enzyme conjugate (12ml)
5. Antibody solution (7ml)
6. Solution A (7ml)
7. Solution B (7ml)
8. Stop solution (7ml)
9. 20×Concentrated wash solution (40ml)
10. 2×Concentrated extraction solution (50ml)

Storage

Storage condition: 2-8°C.
Storage period: 12 months

Performance Characteristics

Accuracy:
Tissue 90±20%
Honey 90±20%
Milk 90±20%

Precision

Variation coefficient of the ELISA kit is less than 10%.

Detection Limit

Tissue: 4ppb
Honey: 0.5ppb
Milk:
Method A 8ppb
Method B & C 4ppb

Sensitivity

Test Sensitivity: 0.2ppb

General Description

Ampicillin belongs to the beta-lactams family, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU, US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5h in single detection, so it can considerably minimize operation error and work intensity.

Standard Curve

1. Percentage absorbance
The mean values of the absorbance values obtained for the standards and the samples are divided by the absorbance value of the first standard (zero standard) and multiplied by 100%. The zero standard is thus made equal to 100% and the absorbance values are quoted in percentages.

B ——absorbance standard (or sample)
B₀ ——absorbance zero standard
2. Standard Curve
To draw a standard curve: Take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the ampicillin standards solution (ppb) as x-axis.
The ampicillin concentration of each sample (ppb), which can be read from the calibration curve, is multiplied by the corresponding dilution factor of each sample followed, and the actual concentration of sample is obtained.
Please notice:
Special software has been developed for all data interpretation, which can be provided on request.