Ampicillin ELISA Kit (DEIA040)

Regulatory status: For research use only, not for use in diagnostic procedures.

Write a review

Size
96T
Sample
Animal tissues, milk, honey
Species Reactivity
N/A
Intended Use
This kit can be used in quantitative and qualitative analysis of ampicillin residue in animal tissue (muscle, liver), honey and milk, etc.
Contents of Kit
1.Microtiter plate with 96 wells coated with antigen
2.Penicillin standard solutions. (1ml×6 bottles)
0 ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb
3.Spiking standard solution: 1ml, 1ppm
4.Enzyme conjugate (12ml)……..…...……………….red cap
5.Antibody solution (7ml) ….………………………green cap
6.Substrate Solution A (7ml) …..………….….…..white cap
7.Substrate Solution B (7ml) ……………...……..…red cap
8.Stop solution (7ml) …………………………...…yellow cap
9.20×Wash concentrate solution (40ml)…..transparent cap
10.2×Concentrated extraction solution (50ml)…...…blue cap
Storage
Store at 2-8°C for 12 months.
Precision
Variation coefficient of the ELISA kit is less than 10%.
Detection Limit
Tissue……………………………………………..…………4ppb
Honey………………………….………………..……..….0.5ppb
Milk:
Method A………………………….………….…………...…8ppb
Method B & C……………………………….…………..…..4ppb
Accuracy
Tissue…………………………………….……….……. 90±20%
Honey ………….…………..……………………………90±20%
Milk………………………………………..…….………. 90±20%
Sensitivity
0.2ppb
General Description
Ampicillin belongs to the beta-lactams family, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU, US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5h in single detection, so it can considerably minimize operation error and work intensity.
Standard Curve
To draw a standard curve: Take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the ampicillin standards solution (ppb) as x-axis.
The ampicillin concentration of each sample (ppb), which can be read from the calibration curve, is multiplied by the corresponding dilution factor of each sample followed, and the actual concentration of sample is obtained.
Please notice:
Special software has been developed for all data interpretation, which can be provided on request.

Citations


Have you cited DEIA040 in a publication? Let us know and earn a reward for your research.

Customer Reviews


Write a review, share your experiences with others and get rewarded !
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket
Product Name Cat. No. Applications Host Species Datasheet Price Add to Basket

References


Thomson, CJ; Jesudason, MV; et al. The prevalence of Vibrio spp. in drinking water and environmental samples in Vellore South India. EPIDEMIOLOGY AND INFECTION 121:67-76(1998).
Woo, PCY; Tsoi, HW; et al. Antibiotics modulate vaccine-induced humoral immune response. CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY 6:832-837(1999).

Online Inquiry

Name:
Phone: *
E-mail Address: *
Technology Interest:
Type of Organization:
Service & Products Interested: *
Project Description:

Related Products

Related Resources

Ordering Information

Payment methods we support:
Invoice / Purchase Order
Credit card

Inquiry Basket