Active Listeriolysin (DAG-P2785)

Active Listeriolysin (aa 26 - 529), recombinant protein from E. coli

Product Overview
Active Listeriolysin (LLO) full length protein
Nature
Recombinant
Tag/Conjugate
Unconjugated
Bio-activity
Activity: this antigen has a hemolytic activity 1.25x106 HU/mg. 2mM DTT is used to reactivate the toxin.
Procedure
1mM EDTA
Purity
> 90 % by SDS-PAGE.Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE
Format
Liquid
Buffer
Preservative: None Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA
Preservative
None
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Introduction
Listeria monocytogenes is the bacterium that causes the infection listeriosis. It is a facultative anaerobic bacterium, capable of surviving in the presence or absence of oxygen. It can grow and reproduce inside the host's cells and is one of the most vir
Antigen Description
Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.
Keywords
hly; hlyA; lisA; LLO; Thiol activated cytolysin; L. monocytogenes LLO; Listeria monocytogenes LLO

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