Anti-ADIPOQ monoclonal antibody

Objectives: The role of ADIPOQ gene variants on weight loss after a dietary intervention remain unclear. The aim of this study was to analyze the effects of rs266729 of the ADIPOQ gene on cardiovascular risk factors and adiposity parameters after adherence to a Mediterranean-type hypocaloric diet. Method: Eighty-three obese patients were studied before and after 12 wk on a Mediterranean-type hypo-caloric diet. Anthropometric parameters and biochemical profiles were measured. The variant of ADIPOQ gene rs266729 was assessed at basal time by polymerase chain reaction at real time. Results: Two genotype groups were realized (CC versus CG + GG). The final genotype distribution was 48 patients CC (57.8%), 30 patients CG (36.2%) and 5 patients GG (6%). After dietary intervention with a moderate calorie restriction and in both genotypes, body mass index (BMI), weight, fat mass, systolic blood pressure, and waist circumference decreased. After dietary intervention and in non-G allele carriers (CC versus CG+ GG), glucose (delta: -6.2 +/- 1.1 versus -2.9 +/- 1.2 mg/dL; P=0.02), total cholesterol (delta:-15.2 +/- 3.1 versus -3.4 +/- 2 mg/dL; P =0.02), low-density lipoprotein cholesterol (delta, -14.9 +/- 3.1 versus -4.9 +/- 1.2 mg/dL; P=0.01), insulin levels (delta, -4 +/- 0.6 versus 0.7 +/- 0.3 UI/L:P=0.01), homeostasis model assessment for insulin resistance (delta, -1.6 +/- 0.4 versus -0.2 +/- 0.4 units; P=0.01), and adiponectin (delta, -10.4 +/- 3.1 versus -1.3 +/- 1.0 ng/dL; P=0.01) improved. Conclusion: After weight loss, the CC genotype of ADIPOQ gene variant (rs266729) is associated with increases in adiponectin levels and decreases of low-density lipoprotein cholesterol, insulin and homeostasis model assessment for insulin resistance after weight loss. (C) 2018 Elsevier Inc. All rights reserved.

The Korean Hanwoo breed possesses a high capacity to accumulate intramuscular fat, which is measured as a marbling score in the beef industry. Unfortunately, the development of marbling is not completely understood and the identification of differentially expressed genes at an early age is required to better understand this trait. In this study, we took muscle samples from 12 Hanwoo steers at the age of 18 and 30 months. From the contrast between age and marbling score, we identified in total 1883 differentially expressed genes (FDR < 0.05 and logarithm fold change >= 1.5) with 782 genes up-regulated and 1101 down-regulated. Differences in gene expression were higher between the ages x marbling groups rather than between high and low marbling groups. At 18 months of age, the genes SLC38A4, ABCA10, APOL6, and two novel genes (ENSBTAG00000015330 and ENSBTAG00000046041) were up-regulated in the high marbling group. From the protein-protein interaction network analysis, we identified unique networks when comparing marbling scores between different ages. Nineteen genes (AGT, SERPINE1, ADORA1, FOS, LEP, FOXO1, FOXO3, ADIPOQ, ITGA1, SDC1, SDC4, ITGB3, ITGB4, CXCL10, ACTG2, MX1, EDN1, ACTA2, and ESPL1) were identified to have an important role in marbling development. Further analyses are needed to better understand the role of these genes.