Intended Use
The Human Zinc-Alpha-2-Glycoprotein ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human zinc-alpha-2-glycoprotein in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
1. ELISA Microplate (Dismountable): 8×12, 2-8°C/-20°C. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard: 2 vials, 2-8°C/-20°C. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Sample Dilution Buffer: 20 ml, 2-8°C
4. Biotin-labeled Antibody(Concentrated): 120 μl, 2-8°C (Avoid Direct Light)
5. Antibody Dilution Buffer: 10 ml, 2-8°C
6. HRP-Streptavidin Conjugate (SABC, 100×): 120 μl, 2-8°C (Avoid Direct Light)
7. SABC Dilution Buffer: 10 ml, 2-8°C
8. TMB Substrate: 10 ml, 2-8°C (Avoid Direct Light)
9. Stop Solution: 10 ml, 2-8°C
10. Wash Buffer (25X): 30 ml, 2-8°C
11. Plate Sealer: 5 pieces
12. Product Description: 1 copy
General Description
Zinc-alpha-2-glycoprotein (ZAG, ZA2G, Azgp1, ZNGP1, Lipid-Mobilizing Factor, LMF) is a soluble 41kDa glycoprotein belonging to the immunoglobulin protein family and consisting of a single polypeptide chain. Human ZAG shares 59% sequence identity with the murine homolog. ZAG is closely related to antigens of the class1 major histocompatibility complex (MHC I) and shares 30-40% sequence identity with the heavy chain of MHC I. Most MHC-I members heterodimerize with beta-2-microglobuline (b2m) and bind peptides derived from intracellular proteins to present them to cytotoxic T cells. In contrast, ZAG is a soluble protein rather than being anchored to plasma membranes that acts independently on b2m and binds the hydrophobic ligand, which may relate to its function in lipid metabolism. ZAG is widespread in body fluids and is also found in various human tissues such as adipose tissue, prostate, breast, skin, salivary gland, trachea, bronchus, lung, gastrointestinal tract, pancreas, liver and kidney. ZAG acts as a lipid mobilizing factor to induce lipolysis in adipocytes and plays an important role in lipid utilization and loss of adipose tissue, especially during cachexia, which occurs in patient suffering from cancer, AIDS and other chronic illnesses. The role of ZAG in cancer cachexia is also connected with its ability to directly influence expression of uncoupling proteins (UCPs), which are implicated in the regulation of energy balance. In human adipocytes, ZAG expression is regulated particularly through TNF alpha and the PPAR gamma nuclear receptor. ZAG expression is also upregulated by glucocorticoids and attenuated by eicosatetraenoic acid (EPA) and beta-3-adrenoreceptor antagonists. ZAG is overexpressed in certain human malignant tumors such as prostate, breast, lung or bladder cancer and can relate to tumor differentiation. Additionally, ZAG plays a role in obesity, diabetic kidney disorders, frontotemporal dementia and regulation of melanin production by melanocytes. ZAG is proposed to have a therapeutic use in obesity and cachexia. It can be used as a marker for clinical analysis of diabetic nephropathy and as a marker for certain tumors.
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
COA
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