Mouse anti-Human ZAK monoclonal antibody, clone 4H6

New polyclonal (pAb) and monoclonal antibodies (mAb) for the immunochemical detection of the anabolic steroids 17 beta-boldenone and methylboldenone have been developed. Enzyme-linked immunosorbent assays (ELISA) were established for the effective control of these illegally applied growth promoters. The IC50 for methylboldenone of pAb 53 was determined 1.69 ng/l providing a linear measuring range in the ELISA between 0.23 and 12.47 ng/l. The IC50 for methylboldenone of pAb 52 was calculated to be 0.54 mg/l with a linear measuring range between 0.03 and 9.54 mg/l. The mAb Bak detects a minimum concentration of 0.13 mg/l 17 beta-boldenone. The IC50 corresponds to 1.6 mg/l. The lower detection limit of mAb Zak was determined at 0.3 mg/l 17 beta-boldenone. The IC50 for this antibody is about 1.2 mg/l. Sample measurements in urine matrices spiked with 2, 3, and 10 mg/l steroid revealed appropriate precision ( average CV for pAb of 4.3%, and for mAb of 10.7%) and recovery rates for the developed ELISAs.

Quasiperiodic structures have recently been found to possess salient topological properties. In this Letter, we theoretically and experimentally demonstrate the topological edge states in a quasiperiodic Harper chain composed of split-ring resonators. In a finite-size Harper chain, we find that there is always a pair of edge states in the bandgaps, independent of the number of the resonators. Different from the topological edge states localized at both ends of a periodic chain, this pair of edge states is selectively localized at the left or right end of the quasiperiodic chain at different frequencies. These paired-edge states with asymmetric field distribution can be used for unidirectional propagation. Our results not only provide a versatile platform to study the topological physics beyond the periodic structures but also may be useful in some applications such as selective unidirectional power transfer. (C) 2018 Optical Society of America