Vitamin B3 ELISA Kit

Sample

Cereals (maize meal, soybean meal, millet flour, rice flour), milk, milk powder

Intended Use

The Vitamin B3 ELISA Kit is used for quantitative analysis of Vitamin B3 in cereals (maize meal, soybean meal, millet flour, and rice flour), milk, milk powder and other food preparations.

Storage

1. All reagents should be stored at 2ºC to 8ºC for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25ºC). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Precision

Intra-Assay: CV<18%
Inter-Assay: CV<20%

Detection Range

12 ng/ml to 192 ng/ml

Detection Limit

It is defined as the lowest detectable concentration corresponding to a signal of Mean of ‘0’ standard plus 2* SD. 10 replicates of ‘0’ standards were evaluated and the LOD was found to be 10 ng/ml.

General Description

Niacin (also known as vitamin B3) is one of the water-soluble B vitamins. Niacin is the generic name for nicotinic acid (pyridine-3-carboxylic acid), nicotinamide (niacinamide or pyridine-3-carboxamide), and related derivatives, such as nicotinamide riboside. Niacin is naturally present in many foods, added to some food products, and available as a dietary supplement.
All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD). More than 400 enzymes require NAD to catalyze reactions in the body, which is more than for any other vitamin-derived coenzyme . NAD is also converted into another active form, the coenzyme nicotinamide adenine dinucleotide phosphate (NADP), in all tissues except skeletal muscle.