Vitamin A (retinol) ELISA Kit

Name: Vitamin A (retinol) ELISA Kit
SKU: DEIASL332
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma and other biological fluids

Detection Method

cELISA

Intended Use

The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of retinol in serum, plasma and other biological fluids.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Pre-coated, ready to use	96-well strip plate, 1	-20°C
2	Plate sealer for 96 wells	4	4°C
3	Standard	2	-20°C
4	Standard Diluent	1×20mL	4°C
5	Detection Reagent A	1×120μL	-20°C
6	Assay Diluent A	1×12mL	4°C
7	Detection Reagent B	1×120μL	-20°C
8	Assay Diluent B	1×12mL	4°C
9	TMB Substrate	1×9mL	4°C
10	Stop Solution	1×6mL	4°C
11	Wash Buffer (30 × concentrate)	1×20mL	4°C

Storage

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
Note:
It is highly recommended to use the remaining reagents within 1 month provided this is prior to the expiration date of the kit. For the expiration date of the kit, please refer to the label on the kit box. All components are stable up to the expiration date.

Performance Characteristics

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level retinol were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level retinol were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/mean × 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Detection Range

123.5-10,000ng/mL. The standard curve concentrations used for the ELISA's were 10,000ng/mL, 3,333.3ng/mL, 1,111.1ng/mL, 370.4ng/mL, 123.5ng/mL.

Sensitivity

The minimum detectable dose of retinol is typically less than 45.1ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by subtracting two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve

In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.