Triazophos [BSA]

This paper reports accurate synthesis of a new type of surface-enhanced Raman spectroscopy (SERS) substrate based on gold nanoparticles decorated 201 red silanized diatomaceous supports. The developed SERS substrate is easy to fabricate, cost effective and offers sensitive rapid detection. The performance and stability of the SERS substrate was investigated and the results showed good SERS activity of substrate that can last for more than 6 months. Tiazophos and phosmet pesticides in aqueous solutions can be detected clearly at a low concentration of 0.01 mg/L. The new substrate was applied to detect triazophos in apple pulp at 0.2 mg/kg. The results indicate that this substrate have good potential in rapid monitoring of pesticide residues in fruits and could be suitable for field-based applications, and routine laboratory analysis of chemicals.

A novel aerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-stain-negative, coccoid to short-rod-shaped bacterial strain, designated YW11(T), was isolated from soil under long-term application of triazophos. The strain was able to hydrolyse triazophos. Strain YW11(T) grew at 15-40 degrees C (optimum at 28 degrees C), at pH 5.0-8.0 (optimum at pH 7.5) and with 0-5.0 % (w/v) NaCl (optimum at 0.5 %). The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were C-18:1 omega 7c, C-16:0, C-18:1 2-OH and C-18:0. The genomic DNA G+C content of strain YW11T was 69.6 +/- 0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, an unknown glycolipid and two unknown aminolipids. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain YW11(T) was a member of the genus Roseomonas, and showed the highest sequence similarity to Roseomonas cervicalis KACC 11686(T) (97.9 %) and Roseomonas aestuaril KACC 19645(T) (97.8%) and then to Roseomonas ludipueritiae KACC 138431 (96.9%). Strain YW11(T). showed low DNA DNA relatedness with R. cervicalis KACC 116861 (32.3 2.9%), R. aestuarii KACC 16549(T) (28.2 2.6%) and R. ludipueritiae KACC 138431 (30.2 2.6 %). Based on the results of phylogenetic analysis and DNA DNA hybridization, the whole-cell fatty acid composition as well as biochemical characteristics, strain YW11(T) was clearly distinguished from all recognized species of the genus Roseomonas and should be assigned to a novel species of the genus Roseomonas, for which the name Roseomonas rhizosphaerae sp. nov. is proposed. The type strain is YVV11(T) (=KACC 17225(T)=CCTCC AB2013041(T)).