Mouse anti-Human TBX5 monoclonal antibody, clone 2H21

Cardiomyogenic differentiation from mesenchymal stem cells has emerged as a novel approach for repair of damaged myocardium. Cell transplantation through direct cell injection is not an optimal method due to the lack of cell-extracellular matrix interactions. In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. The HA/G plasma gels were produced from human blood plasma-derived fibrinogen, gelatin, and human umbilical cord-derived hyaluronic acid. A network-based approach was employed to select marker genes for cardiomyogenic differentiation, and the expression levels of three markers (GATA4, TBX5, and cTnI) were followed by RT-qPCR to investigate the cardiomyogenic differentiation potential of ASCs. Results indicated that each combination of chemical modifiers led to different expression levels in the aforementioned cardiac markers, and this was material-dependent, too. The cardiac gene expression on HA/G plasma gels in the presence of activin-a + BMP-4 or insulin + valproic acid was more pronounced than in the presence of 5-azacytidine only, and scaffold and chemical modifier combinations were crucial for cardiomyogenic differentiation.

Objective of this study was to investigate the difference of retinal structure between C.gariepinus suffer from abnormal pectoral fin and the complete pectoral fins fish. Samples (w=+/- 150g/individual; TL=+/- 27cm) took from the fish farmer from the Leran Village, Manyar Sub-district, District of Gresik, Province of East Java, Indonesia in harvested time (Periode of July-August 2018). HE slides made from left eye organ. There were two groups compared, measured by Dot Slide Scanning Microscope OlyVIA. The variables measured were Total Retinal Thickness, Retinal Pigment Epithelium (RPE), Outer Segment (OS), Middle Layer (ML), and Inner Layer (IL). The results showed that C.gariepinus suffer from abnormal pectoral fin has degenerated Retinal Pigment Epithelium (RPE;72.94 +/- 18.88 mu m), which significantly different (P<0.05) from the normal fish (108.10 +/- 13.29 mu m). Total Retinal Thickness, Outer Segment (OS), Middle Layer (ML), and Inner Layer (IL) in fish suffer from abnormal pectoral fins were remains lower than normal fish but not significantly different. The role of RPE for the retinal function was crucial for homeostasis of the neural retina and visual system. Degenerated RPE will affect their normal function as photoreceptor sheath and reduce the versatility of RPE. From the results, it shows that pectoral fin loss has a relationship with abnormal eyes, and there is morphological integrity between pectoral fins and eyes organ.