Human STAP1 ELISA Kit

Name: Human STAP1 ELISA Kit
SKU: DEIABL527
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cell lysate

Species Reactivity

Human

Intended Use

This Human STAP1 ELISA Kit is used for the quantitative measurement of human STAP1 in cell lysate.
Individual users should determine appropriate conditions when using other types of samples.
This assay kit is for research use only and not for use in diagnostic or therapeutic procedures.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Precision

Intra-assay (Within-Run, n=14) CV=3.7-8.8 %
Inter-assay (Run-to-Run, n=4) CV=3.2-9.3 %

Sensitivity

The limit of detection (defined as such a concentration of human STAP1 giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 65 pg/mL of sample.

General Description

STAP1 (signal transducing adaptor protein 1) also known as BRDG1 (BCR downstream signaling 1), which had been identified as an interacting protein with the kinase domain of TEC tyrosine kinase, appears to function as a docking protein acting downstream of TEC in B cell receptor signaling containing pleckstrin homology, Src homology 2-like domains and a proline-rich region. STAP1 is induced in activated bone marrow-derived macrophages, and by CpG, LPS, and interferon in myeloid cell lines. In addition STAP1 expressing cells show strongly reduced migration.
In 2014, on the other hand, STAP1 was discovered as a fourth gene responsible for autosomal dominant hypercholesterolemia; familial hypercholesterolemia 4 (FH4) following LDLR (FH1), apolipoprotein B (FH2) and PCSK9 (FH3).

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Complete genome sequence analysis of a novel Staphylococcus phage StAP1 and proposal of a new species in the genus Silviavirus

ARCHIVES OF VIROLOGY

Authors: Senevirathne, Amal; Ghosh, Kuntal; Roh, Eunjung; Kim, Kwang-Pyo

Abstract

Bacteriophage StAP1 was isolated from a soil sample infecting Staphylococcus aureus and S. xylosus. Its genome was found to be 135,502 base pairs (bp) long with 30.00 mol% G+C content and 192 open reading frames. While no tRNA encoding genes were identified, 7 mobile elements were found to interrupt five StAP1 open reading frames. Comparative genomic and proteomic analysis consistently supports the establishment of a new species in the genus Silviavirus.

Failure of cosegregation between a rare STAP1 missense variant and hypercholesterolemia

JOURNAL OF CLINICAL LIPIDOLOGY

Authors: Mohebi, Reza; Chen, Qinzhong; Hegele, Robert A.; Rosenson, Robert S.

Abstract

Autosomal dominant familial hypercholesterolemia (FH) is characterized by elevated low-density lipoprotein cholesterol levels and an increased risk for atherosclerotic cardiovascular disease. Although rare pathogenic variants in genes encoding the low-density lipoprotein receptor, apolipoprotein B, proprotein convertase subtilisin/kexin 9 are found in more than 80% of molecularly defined patients with FH, a few rare minor causative genes have been proposed, including the gene encoding signal-transducing adaptor family member 1 (STAP1). Here, we describe a patient with hypercholesterolemia and the rare heterozygous missense variant p.D207N in STAP1. However, extending the pedigree showed failure of the variant to cosegregate with hypercholesterolemia, as both his sons were carriers of the variant and both were also normolipidemic. The findings add to the evidence against STAP1 as a genetic locus for FH. (C) 2020 National Lipid Association. All rights reserved.