Specificity: Reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14.
Species Reactivity: Baboon, Cat, Cow, Dog, Goat, Guinea pig, Human, Horse, Monkey, Pig, Rabbit, Rat
FC (1-2 μg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min, at 4°C) IB (1-2 μg/ml) IF (1-2 μg/ml) IHC (1-2 μg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. or digestion of sections with trypsin at 1mg/ml PBS, 15 min at RT)
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