Product Overview
Recombinant T. gondii ROP4 protein fused to GST tag was expressed in E. coli and purified by proprietary chromatographic technique.
Purity
> 95% pure as determined by 10% PAGE (coomassie staining).
Buffer
25mM Tris-HCl pH 8.0, 5mM Glutathione, 1.5M urea and 50% glycerol.
Storage
2-8°C short term, -20°C long term
Introduction
Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma. The definitive host of is the cat, but the parasite can be carried by many warm-blooded animals (birds or mammals, including humans). Toxoplasmosis, the disease of which is the causative agent, is usually minor and self-limiting but can have serious or even fatal effects on a fetus whose mother first contracts the disease during pregnancy or on an immunocompromised human or cat.
Antigen Description
Of the nine rhoptry proteins identified with available antibodies, only ROP1 and ROP2 have been studied in any detail. ROP1 was initially implicated in invasion as an active component of the penetration-enhancing factor, a poorly characterized cellular fraction that increases the efficiency of host cell invasion by T. gondii. Subsequent gene knockout studies revealed that ROP1 is not essential for either invasion or intracellular survival (21). Although its function remains unknown, ROP1 has been a useful model for studying rhoptry protein trafficking and processing The ROP2 family of proteins (ROP2, ROP3/ROP8, and ROP4) was originally identified by cross-reacting monoclonal antibodies (MAbs) produced against a rhoptry-enriched fraction of T. gondii tachyzoites. Later studies demonstrated that the founding member of this family, ROP2, inserts into the PVM, where it mediates an association between the PV and host cell mitochondria. A recent study using antisense RNA suggested that ROP2 is important not only for the interaction between the PVM and host cell organelles but also for rhoptry biogenesis, parasite invasion, and intracellular replication
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