Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Rat Kim-1 (Kidney Injury Molecule 1) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
62.5-4000 pg/mL
Citations
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YANO, H; MIZOGUCHI, A; et al. THE HERBAL MEDICINE SHO-SAIKO-TO INHIBITS PROLIFERATION OF CANCER CELL-LINES BY INDUCING APOPTOSIS AND ARREST AT THE G(0)-G(1)-PHASE. CANCER RESEARCH 54:448-454(1994).
Rong, S; Park, JK; et al. The TIM-1:TIM-4 Pathway Enhances Renal Ischemia-Reperfusion Injury. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 22:484-495(2011).