Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Rat CD45 (Cluster of Differentiation 45) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.156-10 ng/mL
Citations
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Theil, A; Tuve, S; et al. Adoptive transfer of allogeneic regulatory T cells into patients with chronic graft-versus-host disease. CYTOTHERAPY 17:473-486(2015).
Immig, K; Gericke, M; et al. CD11c-Positive Cells from Brain, Spleen, Lung, and Liver Exhibit Site-Specific Immune Phenotypes and Plastically Adapt to New Environments. GLIA 63:611-625(2015).