Rat anti-Nipah Virus Glycoprotein IgG ELISA Kit

Name: Rat anti-Nipah Virus Glycoprotein IgG ELISA Kit
SKU: DEIA-NIV004
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum or plasma

Species Reactivity

Rat

Detection Method

Indirect-ELISA

Intended Use

The Rat Anti-Nipah Virus Glycoprotein [GP] IgG ELISA Kit is an immunoassay suitable for quantifying IgG antibody activity specific for the glycoprotein of the Nipah virus in serum or plasma of vaccinated, immunized and/or infected hosts.
This immunoassay is suitable for:
• Determining immune status relative to non-immune controls
• Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization, and timing
• Qualifying and standardizing vaccine batches & protocols
The assay is for research use only (RUO) and is not intended nor validated for diagnosing Nipah virus disease. Reagents contain no virus or viral antigens.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Nipah Virus GP Coated Strip Plate	8 × 12	2-8°C
2	Anti-Nipah Virus GP Calibrators	0.65 ml × 4	2-8°C
3	Anti-Nipah Virus GP Positive Control	0.65 ml × 1	2-8°C
4	Low NSB Sample Diluent (LNSD)	30 ml × 1	2-8°C
5	Wash Solution Concentrate (100×)	10ml × 1	2-8°C
6	Sample Diluent Concentrate (20×)	10ml × 1	2-8°C
7	Anti-Rat IgG-HRP Conjugate Concentrate (100×)	0.15ml × 1	2-8°C
8	TMB Substrate	12 ml × 1	2-8°C
9	Stop Solution	12 ml × 1	2-8°C

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

Precision

Intra-assay: <15%
Inter-assay: <15%

General Description

Nipah virus (NiV) is a highly pathogenic member of the genus Henipavirus within the family Paramyxoviridae, originating from fruit bats. NiV was first discovered in Malaysia and Singapore in 1999 during an outbreak of severe respiratory disease in pigs and fatal encephalitis in humans.
Like all paramyxoviruses, NiV is an enveloped virus with a negative-stranded RNA genome. Cell infections start with binding of the viral surface glycoprotein G to cellular ephrin B2 or ephrin-B3 receptors. After receptor binding, the fusion protein, F, mediates pH-independent fusion of the viral envelope with the host cell membrane to allow virus entry (for a review, see reference. While the NiV surface glycoproteins G and F are essential for virus entry processes and later on for cell-to-cell fusion, the third NiV envelope-associated protein, the matrix protein, M, plays an essential role in virus assembly and budding. Similar to many viral matrix proteins, NiV M is a cytoplasmic protein which rapidly associates with cellular membranes. M organizes the assembly of cytoplasmic nucleocapsids and surface glycoproteins at the plasma membrane and is thus needed for efficient release of progeny virus.
Glycoprotein G has been shown to be highly immunogenic in laboratory animals, therefore represents a candidate for effective vaccine development.

Citations