Quinolinic Acid ELISA Kit

Sample

Serum and plasma

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for the quantitative determination of Quinolinic acid in serum and plasma samples. After extraction and derivatization Quinolinic acid is quantitatively determined by ELISA.

Contents of Kit

1. Adhesive Foil, Ready to use, 1 x 4 foils
2. Reaction Plate, Ready to use, 1 x 96 well plate
3. ELISA Wash Buffer Concentrate 50x, Buffer with a non-ionic detergent and physiological pH, 1 x 20 mL/vial, light purple cap
4. Enzyme Conjugate, Ready to use, Goat anti-rabbit immunoglobulins conjugated with peroxidase, 1 x 12 mL/vial, red cap
5. Substrate, Ready to use, Chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide, 1 x 12 mL/black vial, black cap
6. Stop Solution, Ready to use, 0.25 M sulfuric acid Volume, 1 x 12 mL/vial, light grey cap, Hazards identification: H290 May be corrosive to metals.
7. Quinolinic acid Microtiter Strips, Ready to use, 1 x 96 well (12x8), antigen precoated microwell plate in a resealable pouch with desiccant
8. Quinolinic acid Antiserum Concentrate 2x, Rabbit anti-quinolinic acid antibody, blue coloured, 1 x 4 mL/vial, blue cap
9. Quinolinic acid Antiserum Diluent, Ready to use, 1 x 4 mL/vial, orange cap
10. Reaction diluent, Ready to use, 1 x 4 mL/vial, dark green cap
11. Acylation Reagent, Lyophilized, 3 vials, green cap
12. Acylation buffer, Ready to use, 2-(N-Morpholino)ethanesulfonic acid (MES) buffer, 2 x 30 mL/vial, brown cap
13. Standards and Controls, Ready to use, 4 mL/vial
- Standard A, white, 0ng/mL(0nmol/L)
- Standard B, light yellow, 25.08ng/mL(150nmol/L)
- Standard C, orange, 75.25ng/mL(450nmol/L)
- Standard D, dark blue, 225.75ng/mL(1350nmol/L)
- Standard E, light grey, 677.26ng/mL(4050nmol/L)
- Standard F, black, 2031.77ng/mL(12150nmol/L)
- Control 1, light green, Refer to QC-Report for expected value and acceptable range.
- Control 2, dark red, Refer to QC-Report for expected value and acceptable range.
Conversion: Quinolinic acid (ng/mL) x 5.286 = Quinolinic acid (nmol/L)
Contents: Buffer with non-mercury stabilizer, spiked with defined quantity of Quinolinic acid

Storage

Store the unopened reagents at 2 - 8 °C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 1 month when stored at 2 - 8 °C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant again.

Precision

General Description

Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD⁺, a co-factor of many enzymatic reactions.

Standard Curve

Example, do not use for calculation!