Pseudotyped VSV-SARS-CoV-1 S-ΔG-Luciferase

Product Overview

Pseudotyped VSV-SARS-CoV-1 S-ΔG-Luciferase encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with SARS-CoV-1 spike protein. Because the infectivity of Pseudotyped VSV-SARS-CoV-1 S-ΔG-Luciferase is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.The pseudotype VSV particles encode Luciferase together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, Luciferase expression is proportional to the number of cells that were infected.

Nature

Virus

Alternative Names

SARS-CoV-1; Coronavirus; SARS-CoV; SARS-CoV-1 Spike Protein; Severe Acute Respiratory Syndrome; Severe Acute Respiratory Syndrome-1; Severe Acute Respiratory Syndrome glycoprotein; Severe Acute Respiratory Syndrome-1 glycoprotein; SARS-CoV-1 Pseudovirus; SARS-CoV Pseudovirus; SARS Pseudovirus; SARS-CoV-1 RVP; SARS-CoV-1 Reporter Virus Particle; VSV pseudovirus

Application Notes

Neutralization

Storage

Store at -80⁰C . Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80⁰C refrigerator and melt it in an ice bath.

Ship

Frozen on dry ice

Antigen Description

SARS-CoV-1 Envelope Protein

Keywords

SARS-CoV-1; Coronavirus; SARS-CoV; SARS-CoV-1 Envelope Protein; SARS-CoV-1 Envelope glycoprotein; SARS-CoV-1 glycoprotein; Severe Acute Respiratory Syndrome; Severe Acute Respiratory Syndrome-1; Severe Acute Respiratory Syndrome glycoprotein; Severe Acute Respiratory Syndrome-1 glycoprotein; SARS-CoV-1 Pseudovirus; SARS-CoV Pseudovirus; SARS Pseudovirus