Pseudotyped VSV-MERS-CoV S-ΔG-Luciferase encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with SARS-CoV-1 spike protein. Because the infectivity of Pseudotyped VSV-MERS-CoV S-ΔG-Luciferase is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.The pseudotype VSV particles encode Luciferase together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, Luciferase expression is proportional to the number of cells that were infected.
Store at -80⁰C . Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80⁰C refrigerator and melt it in an ice bath.
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