Paraquat ELISA Kit

Sample

serum, plasma or other biological fluids

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for the determination of Paraquat in Samples.

Contents of Kit

1. 96-wells microtiter plate (#S): Twelve strips of 8 detachable wells coated with Anti-Paraquat antibody. 96 (8x12) wells
2. Calibrators: Containing of 0, 0.75, 2.5 and 7.5 ng/mL of Paraquat. 0.5 mL × 4
3. Paraquat-Horseradish Peroxidase Conjugate (PRQ-HRP) (#3), 10.5 mL
4. Tetramethylbenzidine (TMB) substrate (#5): Ready to use. 10.5 mL
5. Wash Buffer (10× PBS-Tween) (#6): Dilute 10 fold with distilled or deionized water to 150 mL prior to use. 15 mL
6. Stop Solution (#7): 3 N HCl. 10.5 mL

Storage

All reagents of the kit are stable, if store at 2-8°C, until the expiration date stated on the kit.

General Description

The herbicide paraquat (1,1'-dimethyl-4,4'-bipuridinium ion) is a potent herbicide, useful for the control of both terrestrial and aquatic weeds. It was also used as preharvest desiccant and defoliant. The use for these purposes requires a sensitive method for the estimation of residues in various crops, soil and water. Estimation of the concentrations of paraquat in biological fluids is a useful diagnostic and in cases of poisoning.