Nicotine is an alkaloid found in the nightshade family of plants (Solanaceae) that acts as a nicotinic acetylcholine agonist and a monoamine oxidase inhibitor. The biosynthesis takes place in the roots and accumulation occurs in the leaves of the Solanaceae. It constitutes approximately 0.6–3.0% of the dry weight of tobacco and is present in the range of 2–7 μg/kg of various edible plants. It functions as an antiherbivore chemical; therefore, nicotine was widely used as an insecticide in the past and nicotine analogs such as imidacloprid are currently widely used.
Keywords
nicotine; L-Nicotine; (-)-Nicotine; (S)-Nicotine
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Q: What is the recommended dilution factor if we were to use DAG3011 for ELISA?
A: For ELISA, the dilution depends on the assay configuration and antibody titer. We recommend starting with a 1:100 dilution.
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