Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of NADP/NADPH content in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer I | 30 mL x 2 | 4°C |
| Reaction Buffer | 10 mL x 1 | 4°C |
| Enzyme | Powder x 1 | -20°C |
| Substrate | 10 mL x 1 | 4°C |
| Dye Reagent A | Powder x 1 | 4°C |
| Dye Reagent B | 1 mL x 1 | 4°C |
| Standard | Powder x 1 | -20°C, keep in dark |
| Instruction Manual | 1 | |
Detection Range
0.5 umol/L - 50 umol/L
General Description
NADP (Nicotinamide adenine dinucleotide phosphate) is a coenzyme composed of ribosyl nicotinamide 5-phosphate (NMN) coupled by pyrophosphate linkage to the 5-phosphate adenosine 2,5-biphosphate. It serves as an electron carrier in several reactions, being alternately oxidized (NADP+) and reduced (NADPH). The oxidative phase of the pentose phosphate pathway is the major source of NADPH in cells, producing approximately 60% of the NADPH required. NADPH provides the reducing equivalents for biosynthetic reactions and the oxidation-reduction involved in protecting against the toxicity of ROS, allowing the regeneration of GSH. NADPH is also used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. NADP/NADPH Microplate Assay Kit provides a simple and direct procedure for measuring NADP+/NADPH levels in a variety of samples. The kit is based on an alcohol dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan reagent. The intensity of the reduced product color, measured at 450 nm, is proportionate to the NADP+/NADPH concentration in the sample.
Citations
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