Microcystin-LR ELISA Kit

Name: Microcystin-LR ELISA Kit
SKU: DEIA3935
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

potable water, surface water samples

Intended Use

The Microcystin-LR ELISA kit is intended for the detection of Microcystin - LR (MC- LR) in environmental samples - potable water and surface water samples.

Contents of Kit

1. ELISA strips (colourless) coated with specific antibody [STRIPS Ab]: 1 microplate
2. Standard A [STANDARD A]: 0.6 mL, 0 ug/L, ready to use, 1 vial
3. Standard B [STANDARD B]: 0.6 mL, 0.1 ug/L, ready to use, 1 vial
4. Standard C [STANDARD C]: 0.6 mL, 0.5 ug/L, ready to use, 1 vial
5. Standard D [STANDARD D]: 0.6 mL, 1 ug/L, ready to use, 1 vial
6. Standard E [STANDARD E]: 0.6 mL, 2.5 ug/L, ready to use, 1 vial
7. MC-LR labeled with horseradish peroxidase [CONJ]: 2.5 mL, ready to use, 1 vial
8. Wash buffer concentrate, 10x concentrated [WASH 10X]: 55 mL, 1 vial
9. Dilution buffer [DIL-MC]: 15 mL, ready to use, 1 vial
10. Chromogenic substrate (TMB substrate) [TMB-BF]: 13 mL, ready to use, 1 vial
11. Stop solution [STOP]: 13 mL, ready to use, 1 vial
12. Instruction manual

Storage

Store the kit reagents at +2 to +10°C, in a dry place and protected from the light. Avoid freezing. Expiration date is indicated at the ELISA kit label and at all reagent labels.
Store unused strips in the sealable pouch and keep the desiccant inside. Transport in thermo bags until 72 hours. Any damages of packaging of kit reagents advise to the producer without delay.
Do not store diluted samples and diluted MC-LR-Px conjugate. Always prepare fresh.

Performance Characteristics

##Validity of the test##
The results of the test are valid if:
The B/Bmax of MC-LR standard ST E is not higher than 50%.
The mean absorbance of MC-LR standard ST A is not lower than 0.700.
The mean B/Bmax of Standards can be lined up as follows ST E < ST D < ST C < ST B < ST A

Precision

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

Ask a Question

Q: We are looking to use this product for recreational water samples. We need to know whether or not our samples should be filtered or not and be stored at what temperature. Also, does this kit test for intracellular and extracellular microcystin?

A: The pre-treatment of the water containing green algae:

The samples are disintegrated with submersible ultrasound Sonoplus according to green algae content (if they are fibrous, disintegration is 3 minutes, 40%. If coccal (Microcystis), then 5 minutes 50-60% ).
Other pre-treatment is not necessary, only dilution with distilled water (if necessary).

The samples should be stored in a fridge at 2-8°C (do not freeze). We recommend to test up to 2 weeks after picking up the samples due to the contamination. We have an experience that the sample had the same reactivity after one year in the fridge (but it should not be contaminated).

Customer Reviews