Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Phosphate content in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Dye Reagent I | 4 mL x 1 | 4°C, keep in dark |
| Dye Reagent II | Powder x 1 | 4°C |
| Dye Reagent II Diluent | 6 mL x 1 | 4°C |
| Standard ( umol/L) | 50 | 1 mL x 1 | 4°C |
| Instruction Manual | 1 | |
Detection Range
1 umol/L - 50 umol/L
General Description
Malachite Green Phosphate Assay Kit provides a fast, reproducible, and non-radioactive method for measuring inorganic free phosphate in aqueous solutions. This simple assay method is based on the complex formed between malachite green molybdate and free orthophosphate under acidic conditions. The formation of the green molybdophosphoric acid complex measured at 635 nm is directly related to the free organic phosphate concentration. Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates. This assay measures only inorganic free phosphate. Lipid-bound or protein-bound phosphates must first be hydrolyzed and neutralized prior to measurement. Overall, this assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate in acidic environments and is amenable to high-throughput screening applications.
Citations
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