Ovotransferrin ELISA Kit

Sample

Plasma, Serum

Species Reactivity

Chicken

Detection Method

sELISA

Intended Use

The Ovotransferrin ELISA Kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of Chickens.

Contents of Kit

1. Diluent Concentrate (Running Buffer): One bottle containing 5× concentrated diluent running buffer. 50 mL
2. Wash Solution Concentrate: One bottle containing 20× concentrated wash solution. 50 mL
3. Enzyme-Antibody Conjugate: One vial containing 100× concentrated affinity-purified anti-Chicken Ovotransferrin antibody conjugated with HRP in stabilizing buffer. 150 μL
4. Chromogenic Substrate Solution: One vial containing 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 12 mL
5. Stop Solution: One vial containing 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 12 mL
6. Anti-Chicken Ovotransferrin ELISA micro plate: Twelve removable eight (8) well strips in well holder frame. Wells are coated with affinity-purified anti-Chicken Ovotransferrin. 96 wells
7. Chicken Ovotransferrin Calibrator: One vial containing Chicken Ovotransferrin Calibrator. 1 vial

Storage

1. Complete Kit: The expiration date for the kit is stated on the outer label.
2. Diluent: The 5× Diluent Concentrate is stable until the expiration date. The 1× working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8°C.
3. Wash Solution: The 20× Wash Solution Concentrate is stable until the expiration date. The 1× working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16-25°C) or at 4-8°C.
4. Enzyme-Antibody Conjugate: Undiluted horseradish peroxidase anti-Ovotransferrin conjugate should be stored at 4-8°C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
5. Chromogen-Substrate Solution: The Substrate Solution should be stored at 4-8°C and is stable until the expiration date.
6. Stop Solution: The Stop Solution should be stored at 4-8°C and is stable until the expiration date.
7. Anti-Chicken Ovotransferrin Elisa Micro Plate: Anti-Chicken Ovotransferrin coated wells are stable until the expiration date, and should be stored at 4-8°C in sealed foil pouch with desiccant pack.
8. Chicken Ovotransferrin Calibrator: Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4°C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.