Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of L-Lactate Dehydrogenase (LDH) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 8 mL x 1 | 4°C |
| Substrate | Powder x 1 | -20°C |
| Dye Reagent A | Powder x 1 | 4°C |
| Dye Reagent B | 1 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
30 umol/L - 600 umol/L
General Description
Lactate dehydrogenase (LDH) is an oxidoreductase present in a wide variety of organisms. It catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD. When disease or injury or toxic material damages tissues, cells release LDH into the bloodstream. Since LDH is a stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. L-Lactate Dehydrogenase Microplate Assay Kit provides a simple and direct procedure for measuring L-lactate dehydrogenase activity in a variety of samples. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color. The rate of increase in the absorbency at 450 nm, is a measure of L-LDH activity.
Citations
Publication ()
Have you cited DFLA26007 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
