Sample
Serum, Plasma, Urine, Saliva, Tissue extracts, Cell lysate, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and Quantification of Iron (Fe3+) in urine, serum, plasma, tissue extracts, cell lysate, and other biological fluid samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reducing Reagent | Powder x 1 | 4°C |
| Reaction Buffer | 5 mL x 1 | 4°C |
| Dye Reagent | Powder x 1 | 4°C |
| Standard ( umol/L) | 500 | 1 mL x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
5 umol/L - 500 umol/L
General Description
Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy. Iron Microplate Assay Kit provides a simple and direct procedure for measuring iron (Fe3+) levels in a variety of samples. Ferrous ions can react with phenanthroline. The product can be measured at a colorimetric readout at 510 nm.
Citations
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