Intrinsic Factor Ab ELISA Kit

Name: Intrinsic Factor Ab ELISA Kit
SKU: DEIA3821
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Plasma, Serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Enzyme Immunoassay for quantitative determination of IgG autoantibodies to intrinsic factor in human serum or plasma.

Contents of Kit

1. Divisible microplate: Consisting of 12 modules of 8 wells each. Ready to use. 96 wells
2. Calibrator A-F (0, 6.3, 12.5, 25, 50, 100 U/mL): Containing serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. 1.5 mL × 6
3. Control positive (1) and negative (2), containing intrinsic factor antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow, Ready to use. The concentration is specified on the certificate of analysis. 1.5 mL × 2
4. Sample Buffer P, containing PBS, BSA, detergent, preservative NaN₃ 0.09%, yellow, 5× conc. 20 mL
5. Enzyme conjugate; containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative ProClin 300 0.05%, light red. Ready to use. 15 mL
6. TMB substrate; containing 3,3',5,5'-Tetramethylbenzidin, colorless. Ready to use. 15 mL
7. Stop solution; contains acid. Ready to use. 15 mL
8. Wash Solution, containing Tris, detergent, preservative NaN₃ 0.09%; 50 × conc. 20 mL

Storage

1. Store the kit at 2-8°C in the dark.
2. Do not expose test reagents to heat, sun or strong light during storage and usage.
3. Store microplate sealed and desiccated in the clip bag provided.
4. Unopened reagents are stable until expiration of the kit. See labels for individual batch.
5. Diluted Wash Solution and Sample Buffer are stable for at least 30 days when stored at 2-8°C.
6. We recommend consumption on the same day.

Performance Characteristics

Calibration: The assay system is calibrated in relative arbitrary units, since no international reference preparation is available for this assay.
Expected values: In a normal range study with samples from healthy blood donors the following ranges have been established with this ELISA assay: Cut-off 6 U/mL

Precision

Intra-Assay precision: Coefficient of variation (CV) was calculated for each of three samples from the results of 24 determinations in a single run. Results for precision-within-assay are shown in the table below.
Inter-Assay precision. Coefficient of variation (CV) was calculated for each of three samples from the results of 6 determinations in 5 different run. Results for run-to-run precision are shown in the table below.

Detection Range

The calculation range of this ELISA assay is 0-100 U/mL

Detection Limit

Functional sensitivity was determined to be: 0.5 U/mL.

General Description

Biermer´s anaemia or pernicious anaemia is the most common cause of vitamin B12 deficiency in Western populations showing the classical features of megaloblastic anemia (i.e. morphologic and functional abnormalities of the blood cells and marrow precursors related to impairment of DNA synthesis). It is characterised by a gastric mucosal defect that decreases the synthesis of intrinsic factor and the occurrence of autoantibodies to gastric parietal cells and to intrinsic factor. Human intrinsic factor is a glycoprotein that is exclusively produced by gastric parietal cells. It plays an essential role in the absorption and transport of vitamin B12 across the small intestine. Two types of intrinsic factor autoantibodies exist. Type I antibodies block the cobalamin binding site on the intrinsic factor molecule, preventing uptake of the vitamin. Type II antibodies block a different site of the intrinsic factor molecule that is involved in binding of the intrinsic factor-cobalamincomplex to ileal receptors. Both types of antibodies have the same pathological effect, i.e. preventing cobalamin resorption by ileal receptors. Serum intrinsic factor autoantibodies can be detected in 50 to 70% of pernicious anaemia patients and are highly specific for Biermer´s anemia with no reported single true positive in a healthy control. Intrinsic Factor Ab ELISA detects both types of autoantibodies and thereby provides a useful tool in the differential diagnosis of pernicious anaemia and other causes of vitamin B12 malabsorption.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

COPYRIGHT PASTE: THE UNFAIRNESS OF STICKING TO TRANSFORMATIVE USE IN THE DIGITAL AGE

UNIVERSITY OF ILLINOIS LAW REVIEW

Authors: Kingsbury, Tim

Abstract

Digital communication continues to transform our world, and information is shared continuously faster because of exponential technological advances. For example, due to an immense online audience, many companies now use GIF images containing short movie or song clips to advertise their business on social media. This has apparently been an effective advertising tool. New information-sharing techniques inevitably birth new copyright implications. Our societal increase in digital communication poses new challenges for copyright jurisprudence and for the courts, because in some instances-such as GIF advertising-it is unclear how current copyright law would distribute certain ownership and usage rights. This Note explores the background and history of U.S. copyright law, including the fair use doctrine, the codification of that doctrine, and the Supreme Court's development of the "transformative use" doctrine. The complex ambiguity of the fair use doctrine is discussed at length, which is emphasized by the inconsistent application of the four fair use factors among the federal circuits. Given the impending confrontation between GIFs and copyright holders, as well as the current ambiguity in fair use doctrine, this Note argues in favor of the Seventh Circuit's return to more traditional copyright values through its appraisal of the "market effects" factor as the most important of the four statutory considerations for determining fair use listed in Section 107 of the Copyright Act of 1976.

Global Existence and Blow-Up for the Fractional p-Laplacian with Logarithmic Nonlinearity

MEDITERRANEAN JOURNAL OF MATHEMATICS

Authors: Boudjeriou, Tahir

Abstract

In this paper, we study the following Dirichlet problem for a parabolic equation involving fractional p-Laplacian with logarithmic nonlinearity ut+(-Delta )psu+|u|p-2u=|u|p-2ulog(|u|)inOmega,t>0,u=0inRN\Omega,t>0,u(x,0)=u0(x),inOmega,where Omega subset of RN(N >= 1) is a bounded domain with Lipschitz boundary and 2 <= p<. The local existence will be done using the Galerkin approximations. By combining the potential well theory with the Nehari manifold, we establish the existence of global solutions. Then by virtue of a differential inequality technique, we prove that the local solutions blow-up in finite time with arbitrary negative initial energy and suitable initial values. Moreover, we give decay estimates of global solutions. The main difficulty here is the lack of logarithmic Sobolev inequality concerning fractional p-Laplacian.