Imidazole ELISA Kit

Sample

Aqueous Samples

Detection Method

cELISA

Intended Use

Imidazole ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of imidazole.

Contents of Kit

1. 96-wells microtiter plate (#S). Twelve strips of 8 detachable wells, coated with Anti-Imidazole antibody: 96 (8×12) wells
2. Calibrator containing 0, 1.0, 3.0 and 9.0 μg/mL of imidazole: 0.9 mL × 4
3. Imidazole-Alkaline Phosphatase conjugate (#3) (IDZ-ALP): 10.5 mL
4. p-Nitrophenyl Phosphate (pNPP) substrate (#5). Ready to use: 10.5 mL
5. Wash Buffer (10×PBS-Tween) (#6). Dilute 10 fold with distilled or deionized water to 150 mL prior to use: 15 mL
6. Stop Solution (#7), 3 N NaOH: 5.5 mL

Storage

All reagents of the kit are stable, if stored at 2-8°C, until the expiration date stated on the kit.

General Description

Imidazole is incorporated into many important biological molecules. The most obvious is the amino acid histidine, which has an Imidazole side chain. One of the applications of Imidazole is in the purification of His-tagged proteins in immobilized metal affinity chromatography (IMAC). Imidazole is used to elute tagged proteins bound to Ni ions attached to the surface of beads in the chromatography column. An excess of Imidazole is passed through the column, which displaces the His-tag from nickel co-ordination, freeing the His-tagged proteins. The Imidazole ELISA Kit can be used to determine quantitatively the concentration of Imidazole in the aqueous sample.

Standard Curve