Hydroxyproline Assay Kit

Sample

Tissue extracts, Cell lysate

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of Hydroxyproline (HYP) content urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.

Contents of Kit

Storage

2 - 8°C

Detection Range

2 umol/L - 200 umol/L

General Description

Hydroxyproline (4-hydroxyproline) is a common non-proteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in several other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. Hydroxyproline Microplate Assay Kit is a simple and sensitive assay to detect small amounts of hydroxyproline in a variety of samples. HYP may react with Chloramine T and DMAB. The products can be measured at a colorimetric readout at 560 nm.