Human sCD25/sIL-2R ELISA Kit

Name: Human sCD25/sIL-2R ELISA Kit
SKU: DEIA9512
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, Plasma, Cell culture supernatant

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The sCD25/sIL-2R ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25/sIL-2R in supernatants, buffered solutions or serum and plasma samples. This assay will recognize both natural and recombinant human sCD25. This kit has been configured for research use only.

Contents of Kit

1. 96 well microtiter strip plate
2. Plastic plate covers
3. Standard: 2200pg/ml
4. Control
5. Standard Diluent (Buffer)
6. Biotinylated anti-CD25
7. Biotinylated Antibody Diluent
8. Streptavidin-HRP
9. HRP Diluent
10. Wash Buffer
11. TMB Substrate
12. H₂SO₄ stop reagent

Storage

Store the kit reagents between 2 and 8°C. Immediately after use, remaining reagents should be returned to cold storage (2-8°C). Expiry of the kit and reagents is stated on box front labels. The expiry of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, the reagent is not contaminated by the first handling.

Precision

The overall intra-assay coefficient of variation has been calculated to be 4.5%.
The calculated overall Inter-assay coefficient of variation was 10.3%.

Sensitivity

The sensitivity or minimum detectable dose of CD25 using this CD25 ELISA kit was found to be 32.5pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments.

Standard Curve

Calculate the average absorbance values for each set of duplicate standards, controls and samples. Ideally duplicates should be within 20% of the mean.
Generate a linear standard curve by plotting the average absorbance of each standard on the vertical axis versus the corresponding CD25 standard concentration on the horizontal axis.
The amount of CD25 in each sample is determined by extrapolating OD values against CD25 standard concentrations using the standard curve.

Note: Curve shown above should not be used to determine results. Every lab must produce a standard curve for each set of microwell strips assayed.
For human serum samples or plasma samples which have been diluted 1:5 according to the protocol, the calculated concentration should be multiplied by the dilution factor (5×).
Standard Calibration:
This immunoassay is calibrated against the International Reference Standard NIBSC 97/600. NIBC 97/600 is quantitated in international units (IU). 1IU corresponding to 57 pg Creative Diagnostics CD25.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Role of CD25 expression on prognosis of acute myeloid leukemia: A literature review and meta-analysis

PLOS ONE

Authors: Li, Jingyuan; Ran, Qijie; Xu, Biao; Luo, Xiaojing; Song, Senhua; Xu, Dehong; Zhang, Xinhua

Abstract

The gene expression for interleukin-2 receptor subunit alpha (CD25/IL2RA) is frequently altered in adults with acute myeloid leukemia (AML). Increasing evidence indicates that the elevated expression of CD25 may be correlated with poor survival for AML patients. Thus, we performed this meta-analysis to further evaluate the prognostic value of elevated CD25 in AML. Eligible studies were gathered by searching on PubMed, Web of Science, and Embase. Using the R language 3.6.0 software, Pooled hazard ratios (HRs) with their corresponding 95% confidence intervals (CIs) of overall survival (OS) and disease-free survival (DFS)/relapse-free survival (RFS)/event-free survival (EFS) for total and subgroup analyses were calculated to investigate the association of elevated CD25 and outcomes of AML patients. Ten studies with a total of 1640 participants were enrolled in this meta-analysis. Pooled HRs suggested that overexpression of CD25 predicted poor outcomes on both OS (HR = 2.27, 95%CI 1.95-2.64) and DFS/RFS/EFS (HR = 1.77, 95%CI 1.44-2.17) in overall population. Subgroup analyses stratified by ethnicity, AML subtype, cut-off value, statistical methodologies and detection method draw similar results. Our meta-analysis indicates that elevated CD25 expression is a poor prognostic factor for AML patients. Considering limited number of samples, further relevant studies are warranted.

Localization of the fourth locus (GLC1E) for adult-onset primary open-angle glaucoma to the 10p15-p14 region

AMERICAN JOURNAL OF HUMAN GENETICS

Authors: Sarfarazi, M; Child, A; Stoilova, D; Brice, G; Desai, T; Trifan, OC; Poinoosawmy, D; Crick, RP

Abstract

One of the major causes of blindness is primary open-angle glaucoma, which affects millions of elderly people worldwide. Genetic studies have so far mapped three loci for the adult-onset form of this condition to the 2cen-q13, 3q21-q24, and 8q23 regions. Herein, we report the localization of a fourth locus, to the 10p15-p14 region, in one large British family with a classical form of normal-tension open-angle glaucoma. Of the 42 meioses genotyped in this pedigree, 39 subjects(lb affected) inherited a haplotype compatible with their prior clinical designation, whereas the remaining 3 were classified as unknown. Although a maximum LOD score of 10.00 at a recombination fraction of theta = .00 was obtained with D10S1216, 21 other markers provided significant values, varying between 3.77 and 9.70. When only the affected meioses of this kindred were analyzed, LOD scores remained statistically significant, ranging from 3.16 (D10S527) to 3.57 (D10S506). Two critical recombinational events in the affected subjects positioned this new locus to a region of similar to 21 cM, flanked by D10S1729 and D10S1664. However, an additional recombination in a 59-year-old unaffected female suggests that this locus resides between D10S585 (or D10S1172) and D10S1664, within a genetic distance of 5-11 cM. However, the latter minimum region must be taken cautiously, because the incomplete penetrance has previously been documented for this group of eye conditions. A partial list of genes that positionally are considered as candidates includes NET1, PRKCT, ITIH2, IL2RA, IL15RA, IT1H2, hGATA3, the mRNA for open reading frame KIAA0019, and the gene for D123 protein.