Human Siglec-9 ELISA Kit

Sample

serum, plasma, cell culture supernatants

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Human Siglec-9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Siglec-9 in serum, plasma, cell culture supernatants and urine.

Contents of Kit

1. Siglec-9 Microplate: 96 wells (12 strips x 8 wells) coated with anti-human Siglec-9.
2. Wash Buffer Concentrate (20X): 25 mL of 20X concentrated solution.
3. Standards: 2 vials of recombinant human Siglec-9.
4. Assay Diluent D: 15 mL diluent buffer for Standard/Sample (serum/plasma) diluent.
5. Assay Diluent B: 15 mL of 5X concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent.
6. Detection Antibody Siglec-9: 2 vial of biotinylated anti-human Siglec-9.
7. HRP-Streptavidin Concentrate: 200 μL 500X concentrated HRP-conjugated streptavidin.
8. TMB One-Step Substrate Reagent: 12 mL of 3, 3', 5, 5'-tetramethylbenzidine (TMB) in buffer solution.
9. Stop Solution: 8 mL of 0.2 M sulfuric acid.

Storage

May be stored for up to 6 months at 2-8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be stored for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. For more detailed information, please download the following document on our website.

Sensitivity

130 pg/mL