Human Prolactin ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Prolactin ELISA kit is used for the quantitative measurement of prolactin in human serum.

Contents of Kit

1. Microwells coated with Streptavidin 12 × 8 × 1
2. Prolactin Standards: 6 vials ( ready to use) 0.5 mL
3. Enzyme Conjugate: 1 bottle (ready to use) 12 mL
4. TMB Substrate: 1 bottle (ready to use) 12 mL
5. Stop Solution: 1 bottle (ready to use) 12 mL
6. 20× Wash concentrate: 1 bottle 25 mL

Storage

1. Store the kit at 2 - 8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.

General Description

Human prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland in both men and woman. Women normally have slightly higher basal prolactin levels than men. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications. Hyperprolactinemia inhibits hypogonadism in men and women with accompanying low FSH and LH levels.

Standard Curve

The standard curve is constructed as follows:
1. Check Prolactin standard values on each standard vial. This value might vary from lot to lot. Make sure you check the value on every kit. See example of the standard attached.
2. To construct the standard curve, plot the absorbance for the standards (vertical axis) versus the standard concentrations (horizontal axis) on a linear graph paper. Draw the best curve through the points.
3. Read the absorbance for controls and each unknown sample from the curve. Record the value for each control or unknown sample.

	OD 450nm	Conc. ng/mL
Std 1	0.037	0
Std 2	0.363	5
Std 3	0.648	10
Std 4	1.181	25
Std 5	1.647	50
Std 6	2.353	100

General Notes

The Prolactin ELISA kit is used for the quantitative measurement of prolactin in human serum.